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Simplified Cannabis Analysis
Analysis by HPLC is the most reliable and accurate method of measuring active components in Cannabis products. SIELC has developed special line of columns – Cannsep, which allows to resolve most of the compounds attributed to cannabis physiological properties. Three columns, Cannsep A, Cannsep B, and Cannsep C resolved all the cannabinoids providing different and significantly orthogonal selectivity. The liquid chromatography does not require high temperature which is the case in gas chromatography (GC) analysis. No thermo-degradation of compounds occurs during the test. In addition to the Cannsep columns, SIELC provides the Alltesta Analyzer, a device that allows for easy and efficient analysis of cannabis products. The Alltesta Analyzer is a user-friendly tool that automates the HPLC testing process and provides accurate and reliable results in a matter of minutes. With the Alltesta Analyzer, you can perform quantitative analysis of components in cannabis products with minimal training and setup time. Columns are available in all standard dimensions and with particles 5 µm for regular HPLC and 3 µm for UPLC (Ultra-high Pressure Liquid Chromatography). If you contract out this test you will pay over $100 per sample. The Cannsep columns are the only consumable that you will ever need to be able to perform such analysis in your own shop or home. One column can be used for a multitude of tests. The incomplete list of compounds that can be analyzed by this HPLC method includes:
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Cannabis Analysis by HPLC Products
Cannsep A
Cannsep A is a reverse-phase analytical column with embedded strong acidic ion-pairing groups. This stationary phase retains neutral cannabinoids through a reverse-phase mechanism. Acidic cannabinoids, such as Tetrahydrocannabinolic acid (THCA) and Cannabidiolic acid (CBDA), will be subject to ion-exclusion interactions with the negatively charged stationary phase of Cannsep A. Mobile phases can be selected for compatibility with low UV detection, using phosphoric or sulfuric acid as modifiers. Alternatively, LC-MS and preparative chromatography compatible mobile phases can be used with formic acid as a modifier. Select options
Cannsep B
Cannsep B is a reverse-phase analytical column with embedded strong basic ion-pairing groups. This stationary phase retains neutral cannabinoids through a reverse-phase mechanism. Acidic cannabinoids, such as Tetrahydrocannabinolic acid (THCA) and Cannabidiolic acid (CBDA), will be subject to ion-exchange interactions with the positively charged stationary phase of Cannsep B. Mobile phases can be selected for compatibility with low UV detection, using phosphoric or sulfuric acid as a modifier. Alternatively, LC-MS and preparative chromatography compatible mobile phases can be used with formic acid as an ionic modifier. Select options
Cannsep C
Cannsep C is a reverse-phase analytical column with a C18 stationary phase and effective end-capping. This stationary phase retains all neutral and acidic cannabinoids through a reverse-phase mechanism. A well-deactivated silica surface and uniform particle distribution provide good peak symmetry and high column efficiency. Mobile phases can be selected for compatibility with low UV detection, using phosphoric or sulfuric acid as a modifier. Alternatively, LC-MS and preparative chromatography compatible mobile phases can be used with formic acid as an ionic modifier. Select options
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